Mao a / B Araf 1 Dxs 426 Pfc Timp Oatl

A combination of Southern blot analysis on a carcinoma DNAs. The breakpoints in these tumors are not the panel of tumor-derived somatic cell hybrids and fluorescence in same, but map within a region of approximately 6.5 kb. situ hybridization techniques was used to map YACs, cosmids Through preparative gel electrophoresis an (X;l) chimaeric and DNA markers from the Xpl 1.2 region relative to the X 4.4-kb EcoRl fragment could be isolated which encompasses chromosome breakpoint of the renal cell carcinoma-associated the breakpoint region present on der(X). Preliminary charact(X;l)(pl l;q21). The position of the breakpoint could be deterterization of this fragment revealed the presence of a 150-bp region with a strong homology to the 5' end of the mouse TFE3 mined as follows: Xcen-OATL2-DXS146~DXS255-SYPt(X;l)-TFE3-OATLl~Xpter. Fluorescence in situ hybridizacDNA in the X-chromosome part, and a 48-bp segment in the tion experiments using TFE3-containing YACs and cosmids chromosome 1-derived part identical to the 5' end of a known revealed split signals indicating that the corresponding DNA EST (accession number R93849). These observations suggest inserts span the breakpoint region. Subsequent Southern blot that a fusion gene is formed between the two corresponding analysis showed that a 2.3-kb ü-VoRI fragment which is present genes in t (X; 1 )(p 11 ;q21)-positive papillary renal cell carin all TFE3 cosmids identified, hybridizes to aberrant restric­ tion fragments in three independent t(X; 1 ̂-positive renal cell "S.